Mario Mandzukic has been left out of Juventus' squad for its Champions League meeting with Barcelona on Tuesday.
Last year's finalist open its European campaign against Barca at Camp Nou, but it will be without Mandzukic who was withdrawn seven minutes from the end of Saturday's 3-0 Serie A victory over Chievo.
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A statement detailing Juve's travelling party explained that Mandzukic had suffered a recurrence of an injury picked up on international duty with Croatia.
New signing Benedikt Howedes will also miss out as he continues a personalised fitness regime, while Giorgio Chiellini, Sami Khedira and Claudio Marchisio remain injured.
Juve met Barca in the quarterfinal of last season's competition, winning 3-0 in Turin before holding the Catalan giant to a goalless draw in the return to seal its progression.
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