Real Madrid forward James Rodriguez and Radamel Falcao not been included in Colombia's squad for next month's friendlies against Brazil and Venezuela.
Madrid confirmed on Monday the 28-year-old had suffered a calf injury in his right leg but did not say how long he would be out for.
James started for the Spanish giant for the first time since May 2017 on Saturday, when Zinedine Zidane's side was held to a 1-1 La Liga draw by Real Valladolid.
Atletico Madrid and Napoli were reportedly interested in signing James during the transfer window, but a deal failed to materialise and it looks likely he will stay at the Santiago Bernabeu beyond the transfer deadline of September 2.
Falcao is yet to make an appearance for Monaco this season due to injury and in his absence Colombia have called up Duvan Zapata, Rafael Santos Borre, Luis Muriel and Roger Martinez in attack for the double header.