Harry Kane scored on his return from injury but Tottenham's Premier League title charge was halted with a 2-1 defeat at Burnley after Ashley Barnes' late strike.
Spurs were looking to add to a run of four consecutive wins and would have moved within a point of leader Manchester City and Liverpool with a victory at Turf Moor on Saturday. But in-form Burnley posed a strong challenge and the Clarets took the lead through Chris Wood's header from a Dwight McNeil corner the visitor felt was incorrectly awarded.
Kane, who was sidelined for six weeks by an ankle problem, appeared to have claimed a point for Spurs but Barnes - who has now scored in four consecutive top-flight outings - turned in decisively from close range with seven minutes to go.
Hugo Lloris looked uncomfortable under a couple of early high balls, while the returning Kane rifled a left-footed drive wide in the 16th minute. As the game opened up, Tom Heaton easily denied Christian Eriksen before Barnes whistled a first-time strike narrowly over the crossbar.
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Kane's swerving 25-yard strike forced a superb save from Heaton after the break but it was the Clarets who took the lead in the 57th minute. McNeil's in-swinging corner from the right wing found Wood rising above the Tottenham defence to nod home his sixth goal in eight Premier League matches.
Mauricio Pochettino sent on Fernando Llorente to join Kane in attack and the England captain levelled just eight minutes after Wood's opener. Rose caught Burnley's defence cold with a clever throw-in that sent Kane through to beat Heaton with a cute finish.
But as Spurs pushed for a winner they were downed by Barnes, who showed a poacher's instinct to arrive at the back post and convert a cross-shot from substitute Johann Gudmundsson.
What does it mean? Spurs' title charge halted
A three-way challenge for the league looked on the cards, with Spurs putting pressure on City and Liverpool thanks to a fine run of results despite the absence of Kane in recent weeks. But defeat at Burnley represents a missed opportunity, especially with City not in league action this weekend - a fact apparently not lost on Pochettino as he raged at referee Mike Dean following the final whistle.
Heaton shines in front of Southgate
Burnley's run of eight league matches without defeat has coincided with the return of club captain Heaton in goal, replacing Joe Hart. He made a wonderful save to deny Kane, which would surely have impressed the watching England manager Gareth Southgate.
A midweek round of fixtures in the Premier League sees Burnley go to Newcastle United in a big game at the bottom of the table. A day later, it's a huge London derby as Chelsea host Tottenham.