Scott McTominay and Andrew Robertson have left the Scotland squad ahead of the final Euro 2020 qualifiers with Cyprus and Kazakhstan.
Bournemouth winger Ryan Fraser has also withdrawn, while Leeds United's Liam Cooper pulled out on Monday.
Midfielders Lewis Morgan and Graeme Shinnie have been added to the squad.
Manchester United midfielder McTominay damaged his ankle in the 3-1 Premier League win over Brighton and Hove Albion and had to be substituted.
The 22-year-old is expected to have tests on the problem later this week.
Scotland cannot qualify for Euro 2020 through Group I as it is 12 points behind top two Belgium and Russia with two games to play.
However, it will be involved in the play-offs in March after finishing top of Nations League C, Group 1.
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